Cell viability as a measure of AAV2 induced cell death in the breast cancer cell lines. (A) MCF-7, (B) MDA-MB-468 and (C) MDA-MB-231 cells were infected with AAV2. Trypan blue exclusion was used to determine the growth inhibitory effect of AAV2 on the monolayer cultures of the different breast cancer cell line cultures. Results shown are representative of three individual experiments. t = time. Arrows indicate days when the cells were passaged at a ratio of 1:2.