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Figure 5 | Molecular Cancer

Figure 5

From: Adeno-associated virus type 2 infection activates caspase dependent and independent apoptosis in multiple breast cancer lines but not in normal mammary epithelial cells

Figure 5

Calcium-phosphate transfection of the cloned AAV2 genome into MCF-7 cells and nHMECs. (A) MCF-7 cells. Untransfected cells, GFP-only controls (+GFP), and GFP vector and AAV2 genome co-transfected cells (+GFP/+AAV2). All images were captured with a 20× objective. (B) MCF-7 cells. Fluorescent Activated Cell Sorting (FACS) Analysis of transfected cells analysed at 48 h and 7 days post-transfection. Percentages of different cell cycle phases are represented in the G1, S and G2 fractions. Percentage denoting "Debris" is indicative of DNA damage induced cell death/loss of membrane integrity. (C) nHMECs. Untransfected cells, GFP-only controls (+GFP), and GFP vector and AAV2 genome co-transfected cells (+GFP/+AAV2). All images were captured with a 20× objective. (D) nHMECs. Fluorescent Activated Cell Sorting (FACS) Analysis of transfected cells analysed at 48 h and 7 days post-transfection. Percentages of different cell cycle phases are represented in the G1, S and G2 fractions.

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Molecular Cancer

ISSN: 1476-4598