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Figure 5 | Molecular Cancer

Figure 5

From: The C-terminal region of Bfl-1 sensitizes non-small cell lung cancer to gemcitabine-induced apoptosis by suppressing NF-κB activity and down-regulating Bfl-1

Figure 5

Bfl-1 siRNA sensitized lung cancer cells to low dose gemcitabine. A , A549 cells were transfected with three types of Bfl-1 siRNA and then treated with 40 ng/ml gemcitabine for 48 h. Cells were then stained with Annexin V-FITC/PI and analyzed by flow cytometry. Experiments were performed three times and yielded comparable results. B, Total protein and RNA were extracted from A549 cells transfected with three types of Bfl-1 siRNA. 50 μg of total cellular proteins were subjected to SDS-PAGE and then immunoblotted using anti-Bfl-1 and -α-tubulin antibodies (upper panel), and RNAs were subjected to RT-PCR to determine Bfl-1 expression. β-actin was used as an internal control (lower panel).

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