The CASP8AP2 / FLASH RNAi signature. (A) Heat map representation of those genes showing a significant fold change (> ± 0.6 Log2 fold change, p < 0.05) 72 hours post transfection of two different siRNAs corresponding to CASP8AP2/FLASH. The 10 genes most altered in expression (up and down) following the silencing of CASP8AP2/FLASH are indicated. Median values are shown where multiple probes corresponding a specific gene were present. The probe corresponding to CASP8AP2/FLASH was the fourth most downregulated gene in the CASP8AP2 expression profile (ranked by changes mediated by siCASP8AP2.3 and then siCASP8AP2.6). The maximum fold changes in expression seen following the silencing of CASP8AP2/FLASH ranged from about a 2-fold linear decrease in expression to an over 20-fold increase in expression of over twenty genes. (B) The enrichment of functional ontologies for those genes upregulated following silencing of CASP8AP2/FLASH. No functional ontologies were significantly for those genes downregulated following CASP8AP2/FLASH loss of function. (C) Gene set enrichment analysis (GSEA) of the targets of transcription factors associated with CRC; (i) shows the overall statistical analysis for the enrichment of the targets within the CASP8AP2/FLASH RNAi signature for six transcription factors; NES refers to the normalized enrichment score, (ii) shows the enrichment plots for the targets of MYC and NFκB.