Figure 1

Cisplatin-mediated hMSH5 protein induction. (A) Time course of hMSH5 protein induction by cisplatin. 293, 293T, and A549 cells were subjected to 20 μM cisplatin and hMSH5 levels were analyzed by Western blotting at the indicated time points. (B) Cisplatin dose-dependent induction of hMSH5 protein in 293, 293T and A549 cells. Cells were treated with 2, 10 and 20 μM cisplatin for 24 hrs, and the levels of hMSH5 were analyzed by Western blotting. (+) Lysates from 293T/f-hMSH5 cells were used as positive controls, and α-tubulin was used as a loading control. (C) Cisplatin-induced hMSH5 induction occurred predominately in the nucleus. 293T and A549 cells were first treated with 10 μM cisplatin for 8 hrs, followed by Western blot analysis of hMSH5 levels in the nuclear and cytoplasmic fractions. RNAPII and α-tubulin were used as nuclear and cytoplasmic markers, respectively.