Figure 4

Mutation of plexinB1 does not affect interaction of plexinB1 with ErbB2 or c-Met. Lysates of HEK293 cells co-expressing ErbB2(A) or c-Met (B) and VSV-plexinB1 (WT or mutant) were immunoprecipitated with anti-VSV and bound proteins detected with anti-ErbB2 (A) or anti c-Met (B). Representative of 3 independent experiments. C). HEK293 cells transfected with plexinB1(WT or mutant) or vector were treated with control or sema4D conditioned medium or with EGF(500 ng/ml) for 20 mins and the phosphorylation of ErbB2 detected by immunoblotting.