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Figure 5 | Molecular Cancer

Figure 5

From: A STAT3-inhibitory hairpin decoy oligodeoxynucleotide discriminates between STAT1 and STAT3 and induces death in a human colon carcinoma cell line

Figure 5

Specific binding of hpdODN B to STAT3 detected by in-cell pull-down. Western-blotting of STAT1 and STAT3 bound to biotinylated hpdODNs after transfection and in-cell pull-down with avidine-coated beads. Cells were either not treated (-) (lanes 1, 2, 3 and 7, 8) or treated (+) (lanes 4, 5, 6 and 9) with IFNγ (50 ng/ml, 16 h), and hpdODN A (lanes 1 and 4), hpdODN B (lanes 3 and 6), hpdODN C (lanes 2 and 5) hpdODN D (lanes 8 and 9) and hpdODN E (lane 7) (hpdODNs were at 1 μg/ml for 16 h). Following cell lysis, protein concentration was measured, identical amounts of protein per sample (250 μg) were added; anti-tubulin western blotting from aliquots of the extracts was performed to show identical loading. Binding experiments were repeated at least three times. All lanes are part of the same blot (identical exposure time) and have been separated for clarity.

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Molecular Cancer

ISSN: 1476-4598