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Figure 1 | Molecular Cancer

Figure 1

From: Klotho-beta overexpression as a novel target for suppressing proliferation and fibroblast growth factor receptor-4 signaling in hepatocellular carcinoma

Figure 1

KLB expression is upregulated in HCC tumors and cell lines. (A) Analysis of KLB gene expression using qRT-PCR in tumor tissue relative to its matched normal tissue after normalizing to GPADH mRNA. Fold change of < 0.5 indicates down-regulation of KLB expression while > 2-fold change indicates KLB up-regulation in tumors. (B) Wilcoxon signed rank test was used to analyze the difference of KLB gene expression in paired normal (Δ) and tumor liver tissues (○). (C) KLB protein expression was determined in Huh7 cells and an independent set of paired HCC lysates. The ratio of KLB between tumor and normal tissue was calculated as (tumor KLB/normal KLB) after normalizing the protein band density to β-actin. (D) The Wilcoxon signed rank test was used to analyze the difference in KLB/β-actin expression between the two groups. (E) KLB mRNA expression in HCC cell lines are expressed as fold changes relative to THLE2 with error bars representing standard deviation (SD) converted to fold changes. (***, P < 0.001 using Student's t-test).

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Molecular Cancer

ISSN: 1476-4598