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Figure 4 | Molecular Cancer

Figure 4

From: Klotho-beta overexpression as a novel target for suppressing proliferation and fibroblast growth factor receptor-4 signaling in hepatocellular carcinoma

Figure 4

Silencing of KLB or FGFR4 increases expression of liver stemness genes. (A) 48 h post siRNA silencing of KLB or FGFR4 in Huh7 cells, AFP gene expression was determined by qRT-PCR. Data indicates fold change in gene expression normalized to scrambled siRNA. Error bars represent SD converted to fold changes. (B) After 72 h, AFP protein expression was also determined using polyclonal anti-AFP antibodies, normalized to β-actin as loading control. (C) CD133 and CD44 expression after KLB-silencing was subsequently determined as described for (A). (D) The effect of the silencing on CD133 protein expression was performed using anti-CD133 antibody. (E) CD133 and CD44 mRNA levels were measured in Huh7 stable clones expressing control shRNA and FGFR4 shRNA after normalizing to GAPDH.

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Molecular Cancer

ISSN: 1476-4598