Skip to main content
Figure 1 | Molecular Cancer

Figure 1

From: c-Myb regulates matrix metalloproteinases 1/9, and cathepsin D: implications for matrix-dependent breast cancer cell invasion and metastasis

Figure 1

Derivation of MDA-MB-231 cells overexpressing c- myb. The MDA-MB-231 cells were transfected with human c-myb cDNA (MYBup) or the myb-less vector (vector) by lipofection. Pools of G418-resistant cells were cloned. (A) c-myb expression was determined in two independent clones by qRT-PCR. GAPDH was used as an internal control. (B) The c-Myb protein in two independent MYBup clones was determined by immunoblotting, and β-actin was used as a loading control. (C) Transactivation by c-Myb was determined by a luciferase assay using p6MBSluc as a reporter. The luciferase activity of each sample was expressed in relative light units and normalized for transfection efficiency according to the β-galactosidase activity. The columns show the average relative luciferase activity from three independent experiments. Error bars indicate standard deviations. Marks * and ** indicate significant (p < 0.05 and p < 0.01, respectively) differences in the relative amounts of c-myb mRNA (A) and in the luciferase activity (C) in the myb-less vector-transfected cells and MYBup cells as determined by the t-test.

Back to article page

Molecular Cancer

ISSN: 1476-4598