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Figure 2 | Molecular Cancer

Figure 2

From: c-Myb regulates matrix metalloproteinases 1/9, and cathepsin D: implications for matrix-dependent breast cancer cell invasion and metastasis

Figure 2

Overexpression of c-Myb increases the migration and invasiveness of MDA-MB-231 cells through the basement membrane extract/Matrigel. (A) The transmigration assay was performed using Cultrex Cell Invasion Assay. MDA-MB-231MYBup and control cells migrating through a microporous membrane towards the chemoattractant (10% FCS)-containing medium were stained with the Calcein-AM. Cell migration was quantified according to the emitted fluorescence using the Synergy HT microplate reader. The columns show the average values of fluorescence from three independent measurements. (B) To determine cell invasiveness, the membrane was covered with a layer of the basement membrane matrix and the experiment was performed as described above. Error bars indicate the standard deviations. Asterisks indicate significant (p < 0.05) differences in the migration (A) and invasion (B) of the myb-less vector-transfected and MYBup cells as determined by the t-test.

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Molecular Cancer

ISSN: 1476-4598