Figure 3

Kinetics of c-Myb-induced migration and Matrigel invasion of MDA-MB-231MYBup cells. Cell migration/invasion in real time was analyzed by the xCELLigence RTCA. The control MDA-MB-231 (wt, vector), and the c-myb-transfected (M2, M5) cells were seeded at a density of 7.5 × 10⁴ per well and cultured in the chambers of CIM-plates. For the cell migration experiments, the membranes were left uncoated (A, B). For monitoring cell invasion, the membranes were coated with either Matrigel (C, D) or collagen I (E, F) as described in the Material and Methods. The bottom chambers were filled with either complete medium containing 10% FCS as a chemoattractant or with serum-free medium (-) in control wells. The charts show the representative outcomes of kinetics analysis of cell migration (A), Matrigel invasion (C) and collagen I invasion (E). The panels (B, D, F) show the average cell indexes at certain time points (6 h for migration and 12 h for invasion) from seven (migration) and five (invasion) independent measurements. Error bars indicate standard deviations. Asterisks indicate significant (p < 0.05) differences in the migration (B) and Matrigel invasion (D) of the myb-less vector-transfected cells and MYBup cells as determined by the t-test.