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Figure 5 | Molecular Cancer

Figure 5

From: c-Myb regulates matrix metalloproteinases 1/9, and cathepsin D: implications for matrix-dependent breast cancer cell invasion and metastasis

Figure 5

siRNA-mediated cathepsin D silencing reduces the migration and invasion of MDA-MB-231MYBup cells. MDA-MB-231MYBup cells were transfected with cathepsin D (CD) or control (ctrl) siRNA as described in the Material and Methods. (A) The level of cathepsin D protein in these cells was determined by immunoblotting. A representative western blot is presented. (B) Migration and invasion activities of the same cells were determined by the xCELLigence RTCA. The chart shows the representative outcomes of the kinetics analysis of cell migration. (C) The average cell indexes at the 6-h (migration, left) and 12-h (invasion, right) time points, respectively, from five independent measurements are shown. Error bars indicate standard deviations. Marks * and ** indicate significant (p < 0.05 and p < 0.01, respectively) differences in the migration/invasion of the cells transfected with cathepsin D siRNA and control siRNA as determined by the t-test.

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