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Figure 6 | Molecular Cancer

Figure 6

From: c-Myb regulates matrix metalloproteinases 1/9, and cathepsin D: implications for matrix-dependent breast cancer cell invasion and metastasis

Figure 6

c-Myb affects cell migration/invasion in murine 4T1 cells similarly as observed in MDA-MB-231 cells. 4T1 cells were transfected with murine c-myb cDNA (M-M5, M-M8) or with the myb-less vector (vector) by lipofection. G418-resistant cells were selected and cloned. (A) c-myb expression in both cell variants (vector and MYBup, respectively) and nontransfected cells (wt) was determined by immunoblotting. The 4T1MYBup variant was tested for migration (B) and invasion (C) activity using the xCELLigence RTCA as described in Figure 3. The panels represent the average cell indexes at certain time points (6 h for migration and 12 h for invasion) from three (collagen I) and four (migration and Matrigel invasion) independent measurements. Asterisks indicate significant (p < 0.05) differences in the migration (B) and Matrigel invasion (C) of the myb-less vector-transfected cells and MYBup cells as determined by the t-test. Error bars indicate standard deviations.

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Molecular Cancer

ISSN: 1476-4598