Figure 2

Time kinetics of GTPase and actin stimulation: (a) Western blot for expression of actin and GTPases - ras, rac1 and rhoA: lane 1- unstimulated control, lane 2- fMLP 0.5 min, lane 3- fMLP 5 min, lane 4- fMLP 10 min, lane 5- fMLP 30 min and lane 6- fMLP 45 min in normal (n = 14) and CML PMNL (n = 22); For ras and rac1 both the patterns of expression, i.e., 21 kd alone or 21 kd along with 25 kd are depicted. (b-e) Representative FCM plots for expression of F-actin and GTPases- Ras, rac1 and rhoA in normal (n = 14) and CML PMNL (n = 22): i) isotype, s) secondary control, a) unstimulated, b) fMLP 0.5 min, c) fMLP 5 min, d) fMLP 10 min, e) fMLP 30 min, and f) fMLP 45 min.