Table 1 Quantitation of GTPases and actin expression
From: RhoA: A therapeutic target for chronic myeloid leukemia
a) Densitometric analysis of Western blots of GTPases - ras, rac1 and rhoA, and actin in normal and CML PMNL | |||||||
|---|---|---|---|---|---|---|---|
Protein | PMNL | Unstimulated | fMLP stimulation | ||||
| Â | Â | Â | 0.5 min | 5 min | 10 min | 30 min | 45 min |
Actin | Normal | 52.89 ± 10.55 | 42.89 ± 9.01 | 42.22 ± 9.48 $ | 46.67 ± 8.90 | 42.44 ± 10.23 | 34.22 ± 8.61 $ |
|  | CML | 39.50 ± 8.74 | 32.70 ± 7.58 $ | 35.80 ± 9.04 | 37.50 ± 8.44 | 32.30 ± 8.47 | 33.00 ± 9.09 $ |
Ras | Normal | 37.80 ± 9.11 | 41.00 ± 8.05 | 46.71 ± 6.52 | 52.43 ± 9.61 | 58.29 ± 8.60 $ | 59.79 ± 10.66 $ |
|  | CML | 37.37 ± 8.90 | 41.10 ± 12.65 | 46.31 ± 13.52 | 45.37 ± 12.60 | 46.04 ± 12.00 # | 47.72 ± 13.93 |
Rac 1 | Normal | 58.22 ± 9.47 | 67.29 ± 9.30 | 70.64 ± 8.1 | 63.00 ± 9.58 | 60.21 ± 8.58 | 49.21 ± 7.44 |
|  | CML | 63.05 ± 11.33 | 69.90 ± 9.11 | 81.17 ± 11.47 $ | 82.36 ± 11.21 $ | 75.77 ± 9.57 $ | 65.14 ± 9.77 |
Rho A | Normal | 62.71 ± 12.45 | 65.14 ± 15.15 | 49.64 ± 10.88 | 43.36 ± 11.46 $ | 38.31 ± 10.79 $ | 24.75 ± 7.44 |
|  | CML | 97.18 ± 14.24 | 94.27 ± 12.61 | 78.14 ± 11.09 | 78.19 ± 10.59 # | 75.59 ± 9.99 # | 78.00 ± 12.01 $# |
b) Median fluorescence channels in flow cytometric estimation of GTPases - ras, rac1 and rhoA, and F-actin in normal and CML PMNL | |||||||
Protein | PMNL | Unstimulated | fMLP stimulation | ||||
| Â | Â | Â | 0.5 min | 5 min | 10 min | 30 min | 45 min |
F-Actin | Normal | 28.53 ± 3.19 | 36.79 ± 2.43 $ | 32.64 ± 2.48 | 34.39 ± 2.06 | 30.52 ± 3.54 | 26.64 ± 3.44 |
|  | CML | 22.75 ± 2.09 | 23.42 ± 2.29 # | 20.58 ± 1.92 # | 20.01 ± 1.89 # | 23.97 ± 3.12 | 24.65 ± 3.34 |
Ras | Normal | 122.21 ± 24.83 | 133.14 ± 17.94 | 180.49 ± 8.65 $ | 179.18 ± 4.86 $ | 169.19 ± 26.33 | 183.75 ± 27.48 |
|  | CML | 99.86 ± 9.76 | 103.51 ± 13.76 | 102.38 ± 12.88# | 117.40 ± 17.25 # | 115.69 ± 15.40 | 126.65 ± 16.27 $ |
Rac 1 | Normal | 176.62 ± 20.81 | 225.95 ± 46.83 | 186.68 ± 30.36 | 194.79 ± 30.48 | 199.84 ± 29.24 | 217.03 ± 25.76 |
|  | CML | 190.82 ± 19.64 | 118.94 ± 10.18 $ | 129.79 ± 13.71 $ | 132.73 ± 12.81 $ | 170.33 ± 22.15 | 158.16 ± 17.09 $# |
Rho A | Normal | 84.45 ± 11.65 | 82.77 ± 10.91 | 67.84 ± 6.92 | 72.79 ± 7.20 | 72.63 ± 10.34 | 65.71 ± 8.58 |
|  | CML | 73.83 ± 11.33 | 60.68 ± 10.14 | 61.33 ± 10.95 | 62.74 ± 11.41 | 76.22 ± 13.57 | 76.33 ± 12.97 |