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Table 1 Quantitation of GTPases and actin expression

From: RhoA: A therapeutic target for chronic myeloid leukemia

a) Densitometric analysis of Western blots of GTPases - ras, rac1 and rhoA, and actin in normal and CML PMNL
Protein PMNL Unstimulated fMLP stimulation
    0.5 min 5 min 10 min 30 min 45 min
Actin Normal 52.89
± 10.55
42.89
± 9.01
42.22
± 9.48 $
46.67
± 8.90
42.44
± 10.23
34.22
± 8.61 $
  CML 39.50
± 8.74
32.70
± 7.58 $
35.80
± 9.04
37.50
± 8.44
32.30
± 8.47
33.00
± 9.09 $
Ras Normal 37.80
± 9.11
41.00
± 8.05
46.71
± 6.52
52.43
± 9.61
58.29
± 8.60 $
59.79
± 10.66 $
  CML 37.37
± 8.90
41.10
± 12.65
46.31
± 13.52
45.37
± 12.60
46.04
± 12.00 #
47.72
± 13.93
Rac 1 Normal 58.22
± 9.47
67.29
± 9.30
70.64
± 8.1
63.00
± 9.58
60.21
± 8.58
49.21
± 7.44
  CML 63.05
± 11.33
69.90
± 9.11
81.17
± 11.47 $
82.36
± 11.21 $
75.77
± 9.57 $
65.14
± 9.77
Rho A Normal 62.71
± 12.45
65.14
± 15.15
49.64
± 10.88
43.36
± 11.46 $
38.31
± 10.79 $
24.75
± 7.44
  CML 97.18
± 14.24
94.27
± 12.61
78.14
± 11.09
78.19
± 10.59 #
75.59
± 9.99 #
78.00
± 12.01 $#
b) Median fluorescence channels in flow cytometric estimation of GTPases - ras, rac1 and rhoA, and F-actin in normal and CML PMNL
Protein PMNL Unstimulated fMLP stimulation
    0.5 min 5 min 10 min 30 min 45 min
F-Actin Normal 28.53
± 3.19
36.79
± 2.43 $
32.64
± 2.48
34.39
± 2.06
30.52
± 3.54
26.64
± 3.44
  CML 22.75
± 2.09
23.42
± 2.29 #
20.58
± 1.92 #
20.01
± 1.89 #
23.97
± 3.12
24.65
± 3.34
Ras Normal 122.21
± 24.83
133.14
± 17.94
180.49
± 8.65 $
179.18
± 4.86 $
169.19
± 26.33
183.75
± 27.48
  CML 99.86
± 9.76
103.51
± 13.76
102.38
± 12.88#
117.40
± 17.25 #
115.69
± 15.40
126.65
± 16.27 $
Rac 1 Normal 176.62
± 20.81
225.95
± 46.83
186.68
± 30.36
194.79
± 30.48
199.84
± 29.24
217.03
± 25.76
  CML 190.82
± 19.64
118.94
± 10.18 $
129.79
± 13.71 $
132.73
± 12.81 $
170.33
± 22.15
158.16
± 17.09 $#
Rho A Normal 84.45
± 11.65
82.77
± 10.91
67.84
± 6.92
72.79
± 7.20
72.63
± 10.34
65.71
± 8.58
  CML 73.83
± 11.33
60.68
± 10.14
61.33
± 10.95
62.74
± 11.41
76.22
± 13.57
76.33
± 12.97
  1. Values represent Mean ± S.E.M.; Normal - n = 14 and CML - n = 22; $ = significantly different as compared to the unstimulated control; # = significantly different as compared to the respective normal PMNL.