Figure 2
From: Loss of HLTF function promotes intestinal carcinogenesis
Expression of Hltf during mouse development. The analysis was done by determining the activity of LacZ in Hltf knockout mice in which the lacZ reporter was fused in-frame with the endogenous Hltf start codon. (A) Whole mount X-gal staining of E8.5 and E9.5 Hltf +/- embryos, demonstrating the specific expression of Hltf in heart (arrows indicate). (B) Whole mount X-gal staining of E10.5 Hltf +/- embryos shows broad expression of Hltf. (C) X-gal staining of tissues collected from E16.5 Hltf +/- embryos. Hltf was found to be widely expressed in many tissues at this developmental stage. (D) X-gal staining of intestine and colon from two-month old Hltf +/- mice. Hltf was identified to be predominately expressed in the crypts as well as in the intestinal epithelial cells in both intestine and colon.