Figure 7

HLTF knockdown induces chromosomal abnormalities in HCT116 colon cancer cells. (A) Representative SKY image of HCT116^scramble cells, showing a stable karyotype with 45 total chromosomes, der(10)t(10;6) (white arrow), der(16)t(8;16) (white arrowhead) and der(18)t(17;18) (yellow arrow), a documented signature for HCT116 parental cells. (B) Representative SKY images of HLTF knockdown HCT116 cells. Several additional chromosomal abnormalities were identified, which include chromosomal fragment (yellow arrowhead), monosomy chromosome 3 (white arrow), der(15) (15;11) (white arrowhead) and der(16) (16;22) (yellow arrow). (C) The number of structural chromosomal abnormalities in HCT116 cells with and without HLTF knockdown. Two groups of cells were analyzed, one from a short term in vitro culture (10 days) and another from subcutaneous tumors that were generated by these cells. The horizontal bars indicate the mean numbers of chromosomal abnormalities in each population of cells. Each data point represents the total number of structural chromosomal rearrangements per metaphase spread after subtraction of the rearrangements (i.e. stable present translocations) found in HCT116 parental cells. (D) Representative SKY images of HLTF knockdown HCT116 cells, demonstrating the presence of high number of trisomies in the tumor cells (indicated by arrows)