Suppression of AKT1/2 or AKT3 decreases SPARC-induced pro-death signaling in temozolomide (TMZ); suppression of AKT1/2 increases colony forming efficiency, and has no effect on survival in TMZ. A, B. Representative Western blots of LN443 cells treated with control siRNA (Csi), AKT1/2 siRNA (AKT1/2si) or AKT 3 siRNA (AKT3si) for 72 hr. Equal numbers of siRNA-treated cells were plated overnight in growth serum, and then treated with 0 (0.1% DMSO control), or 40, 80 or 100 μM TMZ (TMZ) for 2 days before lysing. Arrows indicate ≥ 2-fold increases or decreases due to HSP27 siRNA treatment. Arrows with brackets indicate 30% and 40% reduction of AKT1 and AKT2, respectively. # - Indicates a ≥ 2-fold increase in the ratio of LC-3II/LC-3I. Asterisks indicate ≥ 2-fold increases or decreases due to TMZ treatment. C. Means for colony forming efficiency ± SD for LN443 cells ± AKT1/2 or AKT3 siRNA plating 750 cells. *** p ≤ 0.0003. D. Means for surviving fraction ± SD for LN443 cells ± AKT1/2 or AKT3 siRNA ± increasing concentrations of TMZ plating 750 cells/60-mm dish.