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Figure 8 | Molecular Cancer

Figure 8

From: Inhibition of HSP27 alone or in combination with pAKT inhibition as therapeutic approaches to target SPARC-induced glioma cell survival

Figure 8

Suppression of pAKT in LN443 enhances autophagic signaling, and decreases survival of LN443 cells. A. Representative Western of LN443 cells treated with 0 (0.1% DMSO) or 100 μM TMZ ± AKT inhibitor IV at the concentrations indicated for 2 days before lysing. Arrows indicate ≥ 2-fold increases or decreases due to TMZ treatment. # - Indicates a ≥ 2-fold increase in the ratio of LC-3II/LC-3I. Asterisks indicate ≥ 2-fold increases or decreases due to AKT inhibitor IV treatment. t = trend. B. Means for surviving fraction ± SD for LN443 cells ± AKT inhibitor IV ± TMZ for 1500 cells/60-mm dish. * p = 0.0075. C. Means for surviving fraction ± SD for LN443 cells ± AKT inhibitor IV ± TMZ for 1500 cells/60-mm dish. *, ** p ≤ 0.0003, *** p = 0.03. Plating 3000 cells gave similar results. D. Representative Western of LN443 cells untreated or treated with AKT inhibitor IV at the concentrations indicated for 2 days before lysing at days 4 or 6.

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