Figure 4

Functional effects of MACC1 downregulation on SW620 cells. Inverse correlation between miR-143 and MACC1 expression in CRC. (A) Effectively suppression of MACC1 protein expression by MACC1 siRNA and miR-143 mimics respectively and combinedly. Note the more efficient suppression of MACC1 induced by combined treatment. Suppression of MACC1 simultaneously resulted in (B) significant inhibition of cell growth and (C) cell migration and invasion (100 × magnification) of SW620 cells compared with negative controls. Note the synergistic inhibitory effect induced by combination of MACC1 siRNA and miR-143 mimics, compared with either of them alone (*P < 0.05). The correlation between miR-143 and MACC1 mRNA expression by scatter plot. (D) An inverse correlation was observed in six CRC cell lines (Spearman' s correlation, r = -0.943, P = 0.005), and (E) also observed in another independent panel of 9 paired CRC tissues and adjacent normal tissues (Spearman's correlation, r = -0.48, P = 0.044). Figure is representative of 3 experiments with similar results.