IFN-γ and (RA) co-operate with nicotine to induce the MUC4 promoter. Chromatin IP assays showing the occupancy of E2F1 and STAT1 on the MUC4 promoter in 4 different pancreatic cancer lines. CD18/HPAF-SF (A), ASPC-1 (B), SW1990 (C) and CAPAN2 (D). Cells were treated with nicotine, IFN-γ, IFN-γ in combination with nicotine, RA and RA in combination with nicotine showed increased E2F1 and STAT1 binding on the MUC4 promoter. Sonicated genomic DNA is used for input. C-Fos was used as a negative control. Nonspecific IgG was used as a negative control in pull-down assays.