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Figure 1 | Molecular Cancer

Figure 1

From: Nicotine, IFN-γ and retinoic acid mediated induction of MUC4 in pancreatic cancer requires E2F1 and STAT-1 transcription factors and utilize different signaling cascades

Figure 1

IFN-γ and (RA) co-operate with nicotine to induce the MUC4 promoter. Chromatin IP assays showing the occupancy of E2F1 and STAT1 on the MUC4 promoter in 4 different pancreatic cancer lines. CD18/HPAF-SF (A), ASPC-1 (B), SW1990 (C) and CAPAN2 (D). Cells were treated with nicotine, IFN-γ, IFN-γ in combination with nicotine, RA and RA in combination with nicotine showed increased E2F1 and STAT1 binding on the MUC4 promoter. Sonicated genomic DNA is used for input. C-Fos was used as a negative control. Nonspecific IgG was used as a negative control in pull-down assays.

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Molecular Cancer

ISSN: 1476-4598