Molecular mechanism implicated in Pemetrexed-induced apoptosis in human melanoma cells

Table 1 p53 genotype of employed cell lines

	A375	Hs294T	HT144	MeWo	Calu-3	H1299
TP53	wt	wt	wt	c.772G>A	c.711G>T	100% del.
				c.949C>T

According to COSMIC database from Sanger institute (http://www.sanger.ac.uk/genetics/CGP/cosmic) and “The TP53 web site” (http://p53.free.fr/Database/Cancer_cell_lines/p53_cell_lines.html) the p53 status varies among the employed cell lines. A375, Hs294T and HT144 melanoma cell lines show a wt p53 while MeWo and Calu-3 have puntual mutations that seem to do not compromise its functional activity. On the other hand, H1299 does not show any p53 activity due to its 100% homozygous deletion.

ISSN: 1476-4598