Figure 4

Runx2 increases migration potential of lung cancer cells. (a) Left panel: Runx2 protein was overexpressed in H-1299 cells via lentiviral vector-mediated gene transfer and overexpression levels are shown by Western blot analysis. Right panel: The mRNA levels of BMP-3B in Runx2 overexpressed H1299 cells as examined by qRT-PCR (b) The Runx2 overexpressing H1299 cells or empty vector (control) were seeded in a 96 well cell culture dish in presence or absence of TGFβ (5ng/ml) and examined for changes in cell proliferation via cell proliferation MTT assay by measuring absorbance at 490 nm. (c) Cell migration in H-1299 cells expressing Runx2 or empty vector control was examined in a standard wound/scratch healing assay in response to TGF-β treatment. Representative images at 40X magnification and average (±standard deviation) wound healing response is shown at indicated time points. (* P ≤ 0.05).