Figure 7

Cdk2 inhibition in tumor cells leads to features of senescence. A) BrdU-incorporation and immunofluorescence staining (red), and corresponding DAPI staining (blue), of cultured wild-type (WT), Irbp-Cyclin D1 (D1), and Irbp-Cyclin D1,p53-/- (D1p53-/-) pineal cells, as indicated, after 7 days of treatment with CVT313 (CVT) or an equal volume of DMSO vehicle (Vh). B) Quantitative analysis of percent BrdU-positive cells in wild-type (WT) and Irbp-Cyclin D1 (D1), and Irbp-Cyclin D1,p53-/- (D1p53-/-) pineal cells treated for 7 days with CVT313 (CVT) or vehicle (Vh). Asterisk denotes statistical significance. C) Quantitative analysis of percent BrdU-positive cells in Irbp-Cyclin D1,p18Ink4c-/- tumor cells treated for 7 days with CVT313 (CVT), NSC625987, or vehicle (Vh). Asterisk denotes statistical significance. Double asterisk is for statistically significant decrease in comparison to both other conditions. D) Representative SABG staining of Irbp-Cyclin D1, p53 -/- (D1p53-/-) and Irbp-Cyclin D1, p18Ink4c -/- (D1p18-/-) tumor cells in culture, treated for 7 days with CVT313, NSC625987, or vehicle, as indicated.