Figure 3
From: Altered CXCR3 isoform expression regulates prostate cancer cell migration and invasion
CXCR3 expression in human normal and prostate cancer cell lines. (A) mRNA expression of CXCR3 in RWPE-1, DU-145, PC-3 and LNCaP cells. CXCR3 RNA was normalized to GAPDH RNA levels for that cell line, prior to normalizing to RWPE-1 cells. Histograms represent mean values (+/-s.d.) of more than three separate experiments. (B) mRNA expression of CXCR3A and CXCR3B in RWPE-1, DU-145, PC-3 and LNCaP cells. The concentration was calculated by a standard curve using known amounts of CXCR3A or CXCR3B plasmids. Histogram represent mean values (+/-s.d.) of more than three separate experiments (*P < 0.05 compared to RWPE-1). (C) Ratio of CXCR3A vs. CXCR3B mRNA expression. The calculation was based on the data in (B). DU-145 and PC-3 cells showed higher ratio than RWPE-1 cells. (D) Protein expression of total CXCR3 and CXCR3B in RWPE-1, DU-145, PC-3 and LNCaP cells. (E) Quantification of protein expression in prostate cancer cells. Histogram represent mean values (+/-s.d.) of three separate experiments (*P < 0.05 compared to RWPE-1).