CXCR3 chemokine promoted prostate cancer cell motility and cell invasion. (A) cell motility and (B) cell invasiveness in RWPE-1, DU-145 and PC-3 cells after 5 nM EGF or 1 μg/ml chemokine treatment for 16 hrs. The moving distance (arbitrary units, A.U.) in cell motility assay was measured by the pixel changes in ImageJ after 16 hrs. Cell invasiveness was evaluated by the number of invaded cells after 16 hr treatment in Matrigel invasion chamber system. Histogram represent mean values (+/-s.d.) of more than three separate experiments each in triplicate (*P < 0.05).