Figure 5

CXCR3 chemokine induced cell motility and invasion via PLCβ3 signaling pathway in prostate cancer cells and blocked cell motility by m-calpain activity inhibition in normal cells. (A) PLCβ3 was significantly knocked down in DU-145 cells by siRNA. Protein expression was quantified by ImageJ and normalized to GAPDH. Histogram represent mean values (+/-s.d.) of three separate experiments (**P < 0.05). CXCR3-chemokine-induced (B) cell motility and (C) cell invasiveness in DU-145 cells reduced after PLCβ3 downregulation. Histogram represent mean values (+/-s.d.) of three separate experiments (*P < 0.05, compared to untreated controls; **P < 0.05, compared to siControl group). (D) cAMP amount in RWPE-1, DU-145 and PC-3 cells after chemokine induction. Histogram represent mean values (+/-s.d.) of three separate experiments (*P < 0.05 compared to untreated controls). (E) Calpain activity in RWPE-1, DU-145 and PC-3 cells after chemokine treatment. Calpain activity was quantified by fluorescent intensity of calpain substrate. The signal intensities of untreated samples were set as 1 in each group. Histogram represents mean values (+/-s.d.) of three separate experiments (*P < 0.05 compared to untreated controls). Total calpain activity included both μ-calpain and m-calpain.