Figure 6
From: Altered CXCR3 isoform expression regulates prostate cancer cell migration and invasion
CXCR3B overexpression in DU-145 cells blocked chemokine-induced cell motility and invasion. (A) CXCR3 and CXCR3B protein expression in DU-145 and CXCR3B overexpression cells (**P < 0.05, compared to DU-145). The expression was quantified by ImageJ and normalized to GAPDH. (B) CXCR3A and CXCR3B mRNA expression in DU-145 and CXCR3B overexpression cells (**P < 0.05, compared to DU-145). This showed no CXCR3A mRNA upregulation in CXCR3B overexpression cells. (C) CXCR3B localization after CXCR3B plasmid expression in DU-145 cells. Bar: 20 μm. CXCR3-chemokine-induced (D) cell motility and (E) cell invasiveness in DU-145 cells decreased after CXCR3B expression. Same methods and analyses were used as in Figure 3. Histogram represent mean values (+/-s.d.) of three separate experiments (*P < 0.05, compared to untreated controls; **P < 0.05, compared to DU-145 group).