Figure 7
From: Altered CXCR3 isoform expression regulates prostate cancer cell migration and invasion
CXCR3B upregulation in DU-145 cells inhibited m-calpain activity. (A) Cell motility reduced in DU-145 CXCR3 overexpressing cells after PLCβ3 downregulation. Histogram represent mean values (+/-s.d.) of three separate experiments (*P < 0.05, compared to untreated controls; **P < 0.05, compared to siControl group). Western blot was representative for three separate experiments. (B) cAMP amount increased after chemokine treatment in DU-CXCR3BOX cells but not DU-145 cells. Histogram represent mean values (+/-s.d.) of three separate experiments (*P < 0.05 compared to untreated controls). (C) Calpain activity in DU-145 and DU-CXCR3BOX cells after chemokine treatment. Same methods and analyses were used as in Figure 4E. Histogram represent mean values (+/-s.d.) of three separate experiments (*P < 0.05 compared to untreated controls). Total calpain activity included both μ-calpain and m-calpain.