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Figure 1 | Molecular Cancer

Figure 1

From: Osteoblast-secreted collagen upregulates paracrine Sonic hedgehog signaling by prostate cancer cells and enhances osteoblast differentiation

Figure 1

AA promotes collagen deposition in mixed cultures of MC3T3 pre-osteoblasts and human prostate cancer cells. In this and other figures, mouse MC3T3 pre-osteoblasts were mixed with either human LNCaP prostate cancer cells genetically modified to overexpress Shh (designated LNShh) or vector-transfected LNCaP cells as controls (designated LNCaP). Mixed cells were then seeded onto chamber slides, and cultures were maintained in AA-free α-MEM complete culture medium with or without exogenous AA [50 μg/ml in this and subsequent figures]. Mixed cultures were maintained for either 14 days (A) or 21 days (B) then stained with von Gieson stain for collagen. Bars = 100 μm in A(a, b, d, e); 50 μm in A(c and f); 20 μm in B.

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Molecular Cancer

ISSN: 1476-4598