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Figure 2 | Molecular Cancer

Figure 2

From: Osteoblast-secreted collagen upregulates paracrine Sonic hedgehog signaling by prostate cancer cells and enhances osteoblast differentiation

Figure 2

Organization of matrix collagen fibrils in mixed cultures of MC3T3 pre-osteoblasts and human prostate cancer cells. MC3T3 pre-osteoblasts were mixed with either LNCaP or LNShh cells then seeded onto Thermanox cover slips kept in 24-well plates. Mixed cultures were maintained for either 7 days (A) or 14 days (B) with exogenous AA. Mixed cultures were fixed and processed for TEM, and digital micrographs were captured as described under Materials and Methods. Results are representative of 2–9 TEM images from different grid sections of 1–3 samples per group. Bars = 1 μm (A, B) and 200 nm (C). *denotes cells.

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Molecular Cancer

ISSN: 1476-4598