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Figure 3 | Molecular Cancer

Figure 3

From: Osteoblast-secreted collagen upregulates paracrine Sonic hedgehog signaling by prostate cancer cells and enhances osteoblast differentiation

Figure 3

Size distribution of collagen fibril diameters in mixed cultures of MC3T3 pre-osteoblasts and human prostate cancer cells. MC3T3 pre-osteoblasts were mixed with either LNCaP or LNShh cells then seeded onto Thermanox cover slips and maintained for 21 days with exogenous AA. Mixed cultures were fixed and processed for TEM, and digital images were captured as described under Materials and Methods. (A1, A2) Results are representative of at least 7 TEM images from different grid sections of 3 samples per group. Bars = 200 nm. *denotes cells. (B1, B2) Density plots represent frequency distribution of fibril diameter sizes in mixed cultures. Normal and kernel density curves are superimposed. Data in each density plot were obtained from 500 fibrils randomly selected from 8–14 TEM images from different grid sections of 2 samples per group. **denotes significant difference in median fibril diameter size, P < 0.001.

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Molecular Cancer

ISSN: 1476-4598