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Figure 6 | Molecular Cancer

Figure 6

From: Osteoblast-secreted collagen upregulates paracrine Sonic hedgehog signaling by prostate cancer cells and enhances osteoblast differentiation

Figure 6

AA upregulates paracrine Shh signaling between MC3T3 pre-osteoblasts and LNShh cells. (A and B) Pre-osteoblasts [MC3T3 and M-TAD cells] were mixed with either LNCaP or LNShh cells as indicated and maintained for 7 days with or without exogenous AA. Results are representative of 2–4 assays from 2 independent experiments, each assay done at least in duplicates. (C) Expression of Gli1 in MC3T3 cells cultured with LNShh cells for 14 days in the presence of AA and varying concentrations of DHP were compared. Data are means ± SD of 3 assays from 2 independent experiments, each assay done at least in duplicates. (D) MC3T3 cells were cultured alone for 24 h with or without 1 μg/ml Shh-N and 50 μg/ml AA. Ptc1 expression levels among groups were compared. All values are means ± SD from 2 independent experiments. *, P < 0.05.

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Molecular Cancer

ISSN: 1476-4598