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Figure 1 | Molecular Cancer

Figure 1

From: Interferon-α enhances antitumor activities of oncolytic adenovirus-mediated IL-24 expression in hepatocellular carcinoma

Figure 1

Expression of adenovirus-mediated interleukin-24 mRNA. The hepatocellular carcinoma cell lines (HepG2, MHCC97L, HCCLM3) and the human normal liver cell line L02 were treated with control media (Lane 1), treated with 1000 U/ml IFN-α (Lane 2), infected with SG600-EGFP (Lane 3), or infected with SG600-IL-24 at MOI = 10 (Lane 4). a. Cells were harvested after 24 h, and RNA was isolated and amplified by RT-PCR as described in “Materials and Methods”. Top line: RT-PCR with IL-24 primers. Bottom line: RT-PCR with β-Actin primers. b. Cells were collected 48 h after infection, and protein lysates were isolated and analyzed by western blotting as described in “Materials and Methods”. The HepG2 cell line was used to determine the expression of IL-24 protein after treatment with control, IFN-α, and SG600-EGFP. Top line: IL-24 Bottom line: β-Actin. c. Using samples from the supernatant of treated cells, protein expression levels were determined 24 hours after IFN-α treatment, 24 hours after SG600-EGFP treatment, and 24, 48, and 72 hours (as indicated) after SG600-IL-24 treatment.

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Molecular Cancer

ISSN: 1476-4598