Expression of TLR4 in human OSCC tissues and cell lines. A. Immunohistochemical examination of the expression of TLR4 and MyD88 in OSCC tissues and adjacent non-malignant epithelia. (a) adjacent non-malignant epithelia; (b) OSCC tissue (magnification × 400). B-D. TLR4 and MyD88 in HIOEC and OSCC cell lines were analyzed by RT-PCR ( B), Western blot ( C) and flow cytometry (D). Green line-covered regions represent isotype controls and red regions represent the detection of TLR4 with mAb.