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Figure 1 | Molecular Cancer

Figure 1

From: The isoflavone metabolite 6-methoxyequol inhibits angiogenesis and suppresses tumor growth

Figure 1

Effect of 6-ME on endothelial and cancer cell proliferation. (A) BBCE cells were seeded 24 h before stimulation by FGF (2.5 ng/ml) in the absence or presence of different concentrations of 6-ME. After 48 h cells were again stimulated with FGF (2.5 ng/ml) in the absence or presence of 6-ME and next day cells were counted. (B) HeLA, LnCAP, MCF7 or T24 cells were treated or not with various concentrations of 6-ME. After 48 h 6-ME was added again and 24 h later cells were counted. (C) HUVE cells were serum starved for 6 h in 5% FBS M199 supplemented with heparin and pen/strep. Then cells were stimulated by VEGF (50 ng/ml) for 18 h in the absence or presence of various concentrations of 6-ME. BrdU was added 6 h before the VEGF-induction was complete. Then indirect immunoflourescence was performed and the cells were viewed using Leica IBRE microscope. Graph indicates percentage of BrdU-incorporated cells ± s.d. derived from four independent experiments. ***p < 0.0001, **p = 0.0013, *p = 0.0056.

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