Figure 2

Integrin subunit expression influences the extent of TGFBI adhesion. A, Western blot analysis of RIPA soluble lysates from a panel of ovarian cancer cell lines probed with antibodies against the indicated proteins. SKOV3 TR cells are an in vitro-derived taxol resistant derivative of the SKOV3 parental line. B, Relative adhesion of SKOV3 and PEO1 ovarian cancer cell lines to rTGFBI and rPeriostin. Results of two independent experiments are represented as absorbance at 540 nm, **p < 0.01. C, SKOV3 cell adhesion to fibronectin, rTGFBI, rPOSTN, and vitronectin coated tissue culture plastic in the presence of vehicle or the indicated integrin blocking antibodies. Results of at least three independent experiments were normalized to poly-L-lysine and represented as percent of vehicle treated control.