Figure 7

Suppression of different integrin and ECM components has distinct effects on paclitaxel-induced death in ovarian cancer cells. A, Western blot analysis was performed on SKOV3 cells transfected with either non-target control siRNA, β1 integrin siRNA, or β3 integrin siRNA. Cells were subjected to varying concentrations of paclitaxel for 24 hours followed by flow cytometric analysis of Annexin-V and propidium iodide staining, *p < 0.05, **p < 0.01. B, Cell titre glo cell viability assay performed on non-target control, β1 integrin, or β3 integrin siRNA expressing cells, treated with increasing concentrations of Paclitaxel for 48 hours. **p < 0.01, ***p < 0.001. C, SKOV3 cells were infected with Lentivirus expressing either non-target control shRNA, TGFBI shRNA, or two separate fibronectin shRNA targets. Western blot analysis was performed on RIPA soluble lysates using antibodies to the specified proteins. Cells were treated with 0.3 μM paclitaxel for 24 hours followed by Annexin-V and propidium iodide staining, and subsequently analyzed by flow cytometry. Experiments were performed in triplicate and are represented as fold increase in cells in early apoptosis, **p < 0.01.