Figure 4

Validation of microarray data by qRT-PCR. By using qRT-PCR, miR-150 , miR-210, miR-107 and miR-125b were analyzed in MPs, their donor cells, the recipient cells before and after MP transfer across both leukaemia and breast cancer. Results depict similar trends in gene expressions across samples by both qRT-PCR and microarray. qRT-PCR expression levels were normalized with respect to the endogenous control gene, U6 whereas the microarray expression levels were normalized with respect to the human 5.8 s rRNA (gi555853). Data expressed as fold differences represents the mean ± SEM o f 2 independent experiments conducted in triplicates * p < 0.05