Figure 7
MPs selectively package miRNA genes. Hierarchical clustering analysis of leukaemia and breast cancer (A) resistant cells (VLB100 or DX) versus their MPs (VLBMP or DXMP) show clear differences in the expression of genes between the cells and the MPs depicting some miRNAs are selectively packaged into MPs. (B) Recipient cells resemble the donor after MP co-incubation. Hierarchical clustering analysis of leukaemia and breast cancer sensitive recipient cells (CEM or MCF-7), resistant donor cells (VLB100 or DX) versus the recipient cells cocultured with MPs (CEM + VLBMP or MCF-7 + DXMP) shows that the recipient miRNA gene expression trends follow that of the donor cells after MP co-incubation across both cells types. Heatmaps show the signal intensities of the co-detected miRNAs which have higher expression levels in the MPs whereas similar expression levels in the coculture with respect to its donor cells. The identified miRNAs have a fold change > 1.5 and p- value < 0.06. Green bars reflect downregulated genes and red bars upregulated genes