Figure 4

Combination treatment with PHA-739358 in Ph-positive ALL cells. (A). Pt2 or UCSF02 cells were exposed to 1 μM PHA-739358 for 3 days. After 3 days, the drug was removed and the cells were cultured without PHA-739358. Viability (left panels) and cell numbers (right panels) were measured. (B). Pt2 and UCSF02 cells were treated with the FTI Lonafarnib and PHA-739358 individually or together for 3 days. Cell viability and viable cell numbers were determined by Trypan blue exclusion assay. (C). Cell cycle analysis of Pt2 and UCSF02 treated with FTI alone or combined with PHA-739358 for 48 hours. (*p < 0.05, **p <0.001).