Figure 1

Interaction between CD24 and Lyn. A. Immunoblot analysis for phosphorylation levels of Src, Lyn, Fyn and lck in SW480^CD24 and vehicle control cells. GAPDH served as an internal control. All of these pictures were representatives of three independent experiments. B. The interaction between Lyn and CD24 was examined in SW480^CD24 (lane 2) and SW480^VEC (lane 3) cells. Immunoprecipitation was performed with an anti-CD24 antibody, and normal rabbit immunoglobulin G (Ig G) served as a control. Western blotting was performed with an anti-Lyn antibody. Input (lane 1) is 1 % of the extract from untreated cells. C. CD24 was depleted with CD24 specific siRNA in SW620 cells. Cell lysates were immunoprecipitated with an anti-CD24 antibody and Western blotting was performed with anti-Lyn antibody.