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Figure 2 | Molecular Cancer

Figure 2

From: Lyn is involved in CD24-induced ERK1/2 activation in colorectal cancer

Figure 2

Inhibition of Lyn activation attenuated CD24-induced CRC cell invasion. A. Representative images from the cell invasion assay. SW480CD24 cells or vehicle control cells were seeded into transwell chambers coated with Matrigel and 10 μM PP2 was added 24 h later and kept for 30 min. The cell invasion assay was performed at 24 h after PP2 treatment. CD24 increased the capacity of SW480 cells to migrate through the filters compared with the controls cells (upper). Furthermore, the migration capacity of the SW480CD24 cells that were treated by PP2 (10 μM) was inhibited (bottom). B. Quantification of cell invasion. Invasive activity was determined as the percent invasion of the control SW480 cells through the Matrigel membrane. Values shown are means ± standard errors (SE) for three independent experiments. * P < 0.05 between SW480VEC and SW480CD24 cells; ** P < 0.05 between (SW480CD24 + DMSO) and (SW480CD24 + PP2) cells. C. Western blotting analysis showed the phosphorylation of Lyn was induced by the over-expression of CD24 (lane 2) and the activation of Lyn was inhibited by PP2 (lane 4). GAPDH served as a loading control. Bar graph depicted the quantitative analysis for protein expression and the expression of each protein was normalized to GAPDH. D. Immunofluorescent staining of cells transfected with a pcDNA3.1(+)-CD24 plasmid. SW480 cells were transfected with a CD24 expression plasmid or vehicle control. After 24 h, cells were fixed, permeabilized and stained with 4,6-diamidino-2-phenylindole (DAPI; blue) and Lyn (green). In the cells with overexpressed CD24, Lyn was activated. phospho-Lyn was more intense in the nucleus.

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