Figure 5

The interaction between Lyn and ERK1/2. A. The interaction between Lyn and ERK1/2 was detected in SW480^CD24 and SW480^VEC cells. Cell lysates were immunoprecipitated by an anti-Lyn antibody and assayed by Western blotting with an anti-ERK1/2 antibody. Normal rabbit IgG served as a control. B. SW620 cells were transiently transfected with CD24 siRNA. Cell lysates were immunoprecipitated by an anti-Lyn antibody and assayed by Western blotting analysis with a ERK1/2 antibody. Pictures are representatives of two or three independent experiments with identical results. C. Immunofluorescent staining of SW620 cells transfected with CD24 siRNA. SW620 cells were transiently transfected with CD24siRNA or vehicle control for 24 h. Cytosolic and nucleus location of Lyn (green) and ERK1/2 (red) were examined by primary antibodies followed by FITC-conjugated secondary antibodies and visualized under fluorescent microscopy (original magnification = 630×, Oil). The nucleus was stained with 4, 6-diamidino-2-phenylindole (DAPI; blue). phospo-Lyn and phospho-ERK1/2 signals were greater and intense in the nucleus.