Re-expression of maternally expressed transcripts from chromosome 14q32 following treatment with epigenetic modifiers and its effect on histone acetylation. (A) The expression levels of the maternal (Meg3, Meg8) and paternal (Rtl1, Dio3) genes in normal melanocytes (NHEM) and melanoma cell lines. (B) The expression levels of Meg3 and Meg8 following treatment with 10 μM 5-AZA + 1.5 mM VPA (v) or 10 μM 5-AZA + 3 mM PBA (p) in five different cell lines. (C) The expression level of mir-127 in melanoma cells treated with epigenetic modifiers (10 μM 5-Aza and 3 mM PBA), as assessed by qRT-PCR and normalized relative to untreated cells. (D) ChIP assay of 14PA melanoma cells untreated or treated with 10 μM 5-Aza and 3 mM PBA. The normalized levels of chromosomal DNA at three different loci following immunoprecipitation with anti-acetylated histone 3 in control cells and in cells treated with epigenetic modifiers are shown. Data is represented as mean±SEM, * signifies p < 0.05. (E) The expression level of mir-376a and mir-376c in cells untreated or treated with 10 μM 5-Aza and 3 mM PBA.