The effect of stable expression of mir-376a and mir-376c on the proliferation and migration of melanoma cells. (A) qRT-PCR analysis of mel33B1 melanoma cells after stable transfection with an empty pTER vector or vectors containing either mir-376a (left) or mir-376c (right). The relative expression levels are numerically depicted. (B) The growth of mel33B1 melanoma cell lines transfected with pTER, mir-376a and mir-376c was assessed using the crystal-violet method for 96 h. * signifies p < 0.05, *^ signifies p < 0.08 (C) The growth of mel33B1 melanoma cell lines transfected with pTER, mir-376a and mir-376c was assessed using the Xcellience TM real-time system. (D) The migration of these three cell lines was assessed using the in-vitro transwell method. Representative micrographs of the transwell membrane are seen. This experiment was repeated three times. (E) Cellular migration was assessed using the Xcellience TM real-time system within a 24 h period.