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Figure 3 | Molecular Cancer

Figure 3

From: Inhibition of Phosphodiesterase-4 (PDE4) activity triggers luminal apoptosis and AKT dephosphorylation in a 3-D colonic-crypt model

Figure 3

Formation of luminal cavities and tight junctions by PDE4 inhibitor in HCT116 cells grown in 3 DC. (A) The signals for ZO-1 and E-cadherin in HCT116 cells treated with DMSO alone or rolipram at day 6 in 3 DC. Nuclear DNA, blue; ZO-1, green; E-cadherin, red. Dots represent luminal cavities. Scale bar = 50 μm. (B) The ratios of 3-D structures with luminal cavity (left panel) and apical ZO-1 signal (right panel). White bar represents a relative intensity of the signal for 3 DC HCT116 cells treated with rolipram, normalized by the signal for 3 DC HCT116 cells treated with DMSO alone (black bar). *, P < 0.0001. (C) Western blotting of ZO-1 for 3 DC HCT116 cells treated with DMSO alone or rolipram at Day 6 (lower panel) and the quantitative analysis of ZO-1 expression level (upper panel). White bar represents a relative intensity of the signal for 3 DC HCT116 cells treated with rolipram normalized by the signal for 3 DC HCT116 cells treated with DMSO alone (black bar). n.s., not significant.

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