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Figure 2 | Molecular Cancer

Figure 2

From: Silencing of Apoptosis-Inducing factor and poly (ADP-ribose) glycohydrolase reveals novel roles in breast cancer cell death after chemotherapy

Figure 2

Chemotherapeutic treatment of human breast adenocarcinoma cells after the silencing of AIF. Cells were transfected with siRNA for 48 h as before. A, MCF-7 cells were then treated with 0.5 mM MNNG x45 min. B, MDA-MB-231 cells were then treated with MNNG or 25 J/m2 UV-C radiation. After 24 h, cell death was quantified by FACS after propidium iodide (PI)/annexin V-FITC staining. C, MDA-MB-231 cells were treated with 20 μM epirubicin (EPI) x6 h, 10 mM cyclophosphamide (CPA) x24 h, or 0.5 mM cisplatin (DDP) x6 h after siRNA transfection. Cell death was then quantified by FACS after PI/annexin staining. For A-C, *P < 0.01 between Scr and AIF (one-way ANOVA and unpaired Student’s t test). Error bars represent the SEM. D, Analysis of nuclear AIF levels in siRNA transfected cells treated with EPI. Con, untransfected cells; Scr, scrambled siRNA transfected cells. All experiments were repeated at least twice with similar results.

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Molecular Cancer

ISSN: 1476-4598