Skip to main content
Figure 3 | Molecular Cancer

Figure 3

From: Silencing of Apoptosis-Inducing factor and poly (ADP-ribose) glycohydrolase reveals novel roles in breast cancer cell death after chemotherapy

Figure 3

Silencing of poly(ADP-ribose) glycohydrolase (PARG) by siRNA in MDA-MB-231 cells. MDA-MB-231 cells were transfected with siRNA oligos for PARG as previously reported [38]. A, Immunoblotting detection of PARG in cell extracts 48 h after transfection using polyclonal anti-PARG antibody (Millipore) (1:2,000 dilution). Equivalent controls wereutilized as in Fig. 1A. B, Densitometric quantification of PARG protein bands from A. *P < 0.01 between Scr and AIF (one-way ANOVA and unpaired Student’s t test). Error bars represent the SEM. C, MDA-MB-231 cells were transfected with PARG siRNA oligos, treated with 0.5 mM MNNG, then analyzed for levels of PAR by immunoblot from 0.5-24 h. Equal protein loading per lane was verified by immunoblotting detection of β-actin. D, Densitometric quantification of PAR levels from C. *P < 0.01 between Scr and PARG (one-way ANOVA and unpaired Student’s t-test). Error bars represent the SEM.

Back to article page

Molecular Cancer

ISSN: 1476-4598